PrimePCR™ Probe Assay: KCND1, Human

print
RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050165
Assay Design:   exonic
Chromosome Location:   X:48822587-48822711question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000334025 ENST00000303415 ENST00000381583 ENST00000290607 ENST00000284674 ENST00000407004 ENST00000589998 ENST00000592763 ENST00000341083 ENST00000376477 ENST00000218176 ENST00000419374 ENST00000318443 ENST00000561213 ENST00000261842 ENST00000560508 ENST00000084798 ENST00000600735 ENST00000593940 ENST00000596377

Gene Information

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release heart rate insulin secretion neuronal excitability epithelial electrolyte transport smooth muscle contraction and cell volume. Four sequence-related potassium channel genes - shaker shaw shab and shal - have been identified in Drosophila and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel voltage-gated shal-related subfamily members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This gene is expressed at moderate levels in all tissues analyzed with lower levels in skeletal muscle. [provided by RefSeq Jul 2008]

Gene Symbol:   KCND1
Gene Name:   potassium voltage-gated channel, Shal-related subfamily, member 1
Aliases:   KV4.1
RefSeq:   NC_000023.10 NG_012515.1 NG_016263.1 NT_079573.4
Ensembl:   ENSG00000102057
Entrez:   3750
UniGene:   Hs.55276
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.840000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.