PrimePCR™ Probe Assay: NFATC4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NFATC4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NFATC4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050096
Assay Design:   exonic
Chromosome Location:   14:24845572-24845697question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000413692 ENST00000554591 ENST00000555590 ENST00000554966 ENST00000424781 ENST00000539237 ENST00000556279 ENST00000553469 ENST00000554050 ENST00000250373 ENST00000553708 ENST00000553879 ENST00000554344 ENST00000554661 ENST00000556169 ENST00000557451 ENST00000422617 ENST00000555453 ENST00000554473 ENST00000556759 ENST00000555167 ENST00000557767 ENST00000555393 ENST00000555802

Gene Information

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family of nuclear factors of activated T cells also participate in the formation of this complex. The product of this gene plays a role in the inducible expression of cytokine genes in T cells especially in the induction of the IL-2 and IL-4. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Oct 2008]

Gene Symbol:   NFATC4
Gene Name:   nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
Aliases:   NF-ATc4, NFAT3
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100968
Entrez:   4776
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.950000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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