PrimePCR™ Probe Assay: VRK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: VRK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VRK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050080
Assay Design:   exonic
Chromosome Location:   14:97326903-97327050question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000216639 ENST00000557222 ENST00000557352

Gene Information

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells such as those in testis thymus fetal liver and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and in vitro to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene therefore may regulate cell proliferation. This protein also phosphorylates histone casein and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq Jul 2008]

Gene Symbol:   VRK1
Gene Name:   vaccinia related kinase 1
Aliases:   MGC117401, MGC138280, MGC142070, PCH1
RefSeq:   NC_000014.8 NG_016293.1 NT_026437.12
Ensembl:   ENSG00000100749
Entrez:   7443
Chromosome Mapping:   14q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.790000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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