PrimePCR™ Probe Assay: DERL3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DERL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DERL3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049989
Assay Design:   exonic
Chromosome Location:   22:24180621-24180914question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000409762 ENST00000409582 ENST00000429174 ENST00000413539 ENST00000258104 ENST00000409651 ENST00000394120 ENST00000409744 ENST00000409366 ENST00000410020 ENST00000410041 ENST00000221086 ENST00000526292 ENST00000325083 ENST00000517730 ENST00000519253 ENST00000524226 ENST00000406855 ENST00000404056 ENST00000318109 ENST00000476077

Gene Information

The protein encoded by this gene belongs to the derlin family and resides in the endoplasmic reticulum (ER). Proteins that are unfolded or misfolded in the ER must be refolded or degraded to maintain the homeostasis of the ER. This protein appears to be involved in the degradation of misfolded glycoproteins in the ER. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Oct 2008]

Gene Symbol:   DERL3
Gene Name:   Der1-like domain family, member 3
Aliases:   C22orf14, IZP6, LLN2, MGC71803, derlin-3
RefSeq:   NC_000022.10 NG_009303.1 NT_011520.12
Ensembl:   ENSG00000099958
Entrez:   91319
Chromosome Mapping:   22q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.600000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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