PrimePCR™ Probe Assay: WAC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: WAC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WAC, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049953
Assay Design:   exonic
Chromosome Location:   10:28824496-28824605question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000375664 ENST00000526722 ENST00000375646 ENST00000347934 ENST00000354911 ENST00000420266 ENST00000442148 ENST00000448193 ENST00000414108 ENST00000428935

Gene Information

The protein encoded by this gene contains a WW domain which is a protein module found in a wide range of signaling proteins. This domain mediates protein-protein interactions and binds proteins containing short linear peptide motifs that are proline-rich or contain at least one proline. This gene product shares 94% sequence identity with the WAC protein in mouse however its exact function is not known. Alternative splicing results in multiple transcript variants. [provided by RefSeq Dec 2008]

Gene Symbol:   WAC
Gene Name:   WW domain containing adaptor with coiled-coil
Aliases:   BM-016, MGC10753, PRO1741, Wwp4, bA48B24, bA48B24.1
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000095787
Entrez:   51322
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.190000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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