PrimePCR™ Probe Assay: WAC, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049953
Assay Design:   exonic
Chromosome Location:   10:28824496-28824605question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000375664 ENST00000526722 ENST00000375646 ENST00000347934 ENST00000354911 ENST00000420266 ENST00000442148 ENST00000448193 ENST00000414108 ENST00000428935

Gene Information

The protein encoded by this gene contains a WW domain which is a protein module found in a wide range of signaling proteins. This domain mediates protein-protein interactions and binds proteins containing short linear peptide motifs that are proline-rich or contain at least one proline. This gene product shares 94% sequence identity with the WAC protein in mouse however its exact function is not known. Alternative splicing results in multiple transcript variants. [provided by RefSeq Dec 2008]

Gene Symbol:   WAC
Gene Name:   WW domain containing adaptor with coiled-coil
Aliases:   BM-016, MGC10753, PRO1741, Wwp4, bA48B24, bA48B24.1
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000095787
Entrez:   51322
UniGene:   Hs.435610
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.190000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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