PrimePCR™ Probe Assay: ZBTB25, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049865
Assay Design:   exonic
Chromosome Location:   14:64957183-64966174question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000357162 ENST00000358544 ENST00000395996 ENST00000374139 ENST00000555220 ENST00000555424 ENST00000261683 ENST00000394715 ENST00000331110 ENST00000374266 ENST00000270812

Gene Information

Description Not Available

Gene Symbol:   ZBTB25
Gene Name:   zinc finger and BTB domain containing 25
Aliases:   C14orf51, KUP, ZNF46
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000089775
Entrez:   7597
UniGene:   Hs.654571
Chromosome Mapping:   14q23-q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.760000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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