PrimePCR™ Probe Assay: RPH3A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RPH3A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RPH3A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049856
Assay Design:   exonic
Chromosome Location:   12:113285561-113303234question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000548197 ENST00000547686 ENST00000543106 ENST00000551593 ENST00000551748 ENST00000546703 ENST00000547840 ENST00000547728 ENST00000549769 ENST00000552667 ENST00000389385 ENST00000550901 ENST00000551198 ENST00000551052 ENST00000415485 ENST00000553114 ENST00000420983 ENST00000380637 ENST00000380634 ENST00000458295 ENST00000380631 ENST00000333634 ENST00000452550

Gene Information

Exocytosis of neurotransmitters and hormones is fundamental to synaptic neurotransmission and cell-cell communication. RAB3A (MIM 179390) is a small G protein that is thought to act at late stages of exocytosis and RPH3A is a RAB3A effector (Lin et al. 2007 [PubMed 17149709]).[supplied by OMIM Jul 2008]

Gene Symbol:   RPH3A
Gene Name:   rabphilin 3A homolog (mouse)
Aliases:   KIAA0985
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000089169
Entrez:   22895
Chromosome Mapping:   12q24.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.430000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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