PrimePCR™ Probe Assay: GP6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GP6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GP6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049836
Assay Design:   exonic
Chromosome Location:   19:55539101-55543511question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000372371 ENST00000327928 ENST00000417454 ENST00000310373 ENST00000333884 ENST00000468239 ENST00000575496 ENST00000570488 ENST00000571871 ENST00000574687 ENST00000571499 ENST00000570691 ENST00000573764 ENST00000575607 ENST00000572481 ENST00000573560 ENST00000571588 ENST00000577014 ENST00000572462 ENST00000576196 ENST00000575740 ENST00000575021 ENST00000574117 ENST00000575642 ENST00000572667 ENST00000571718 ENST00000572758 ENST00000573444 ENST00000574603 ENST00000577116 ENST00000572791 ENST

Gene Information

This gene encodes a platelet membrane glycoprotein of the immunoglobulin superfamily. The encoded protein is a receptor for collagen and plays a critical role in collagen-induced platelet aggregation and thrombus formation. The encoded protein forms a complex with the Fc receptor gamma-chain that initiates the platelet activation signaling cascade upon collagen binding. Mutations in this gene are a cause of platelet-type bleeding disorder-11 (BDPLT11). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Dec 2011]

Gene Symbol:   GP6
Gene Name:   glycoprotein VI (platelet)
Aliases:   GPIV, GPVI, MGC138168
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000088053
Entrez:   51206
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.240000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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