PrimePCR™ Probe Assay: SF3B2, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049827
Assay Design:   exonic
Chromosome Location:   11:65822547-65822745question
Amplicon Length:   169
Splice Variants Targeted:   ENST00000566339 ENST00000301717 ENST00000542440 ENST00000267197 ENST00000528302 ENST00000322535 ENST00000524627 ENST00000533595 ENST00000530322 ENST00000524475 ENST00000483722 ENST00000545968 ENST00000399249 ENST00000256993 ENST00000381569 ENST00000265801 ENST00000538941 ENST00000563290 ENST00000324767 ENST00000356924 ENST00000556517 ENST00000298816 ENST00000544564 ENST00000541571 ENST00000535424 ENST00000400004 ENST00000400003 ENST00000329538 ENST00000524189 ENST00000393119 ENST00000313863 ENST

Gene Information

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b together with splicing factor 3a and a 12S RNA unit forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq Jul 2008]

Gene Symbol:   SF3B2
Gene Name:   splicing factor 3b, subunit 2, 145kDa
Aliases:   Cus1, SAP145, SF3B145, SF3b1, SF3b150
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000087365
Entrez:   10992
UniGene:   Hs.406423
Chromosome Mapping:   11q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.320000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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