PrimePCR™ Probe Assay: HSD17B14, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HSD17B14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HSD17B14, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049816
Assay Design:   exonic
Chromosome Location:   19:49316386-49316534question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000263278 ENST00000596349 ENST00000599157 ENST00000595764

Gene Information

17-beta-hydroxysteroid dehydrogenases such as HSD17B14 are primarily involved in metabolism of steroids at the C17 position and also of other substrates such as fatty acids prostaglandins and xenobiotics (Lukacik et al. 2007 [PubMed 17067289]).[supplied by OMIM Jun 2009]

Gene Symbol:   HSD17B14
Gene Name:   hydroxysteroid (17-beta) dehydrogenase 14
Aliases:   DHRS10, SDR47C1, retSDR3
RefSeq:   NC_000019.9 NG_013003.1 NT_011109.16
Ensembl:   ENSG00000087076
Entrez:   51171
Chromosome Mapping:   19q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 38.540000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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