PrimePCR™ Probe Assay: MPP4, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049739
Assay Design:   exonic
Chromosome Location:   2:202557739-202558684question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000409474 ENST00000315506 ENST00000396886 ENST00000428900 ENST00000409143 ENST00000447335 ENST00000602867 ENST00000359962

Gene Information

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) protein family with an N-terminal PDZ domain a central src homology 3 region (SH3) and a C-terminal guanylate kinase-like (GUK) domain. The protein is localized to the outer limiting membrane in the retina and is thought to function in photoreceptor polarity and the organization of specialized intercellular junctions. Alternatively spliced transcript variants have been described but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   MPP4
Gene Name:   membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4)
Aliases:   ALS2CR5, DLG6
RefSeq:   NC_000002.11 NG_008775.1 NG_012654.1 NT_005403.17
Ensembl:   ENSG00000082126
Entrez:   58538
UniGene:   Hs.63085
Chromosome Mapping:   2q33.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 37.320000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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