PrimePCR™ Probe Assay: CDC14B, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049731
Assay Design:   exonic
Chromosome Location:   9:99285545-99285656question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000375241 ENST00000375240 ENST00000375242 ENST00000463569 ENST00000265659 ENST00000375236 ENST00000520309 ENST00000313581 ENST00000431575

Gene Information

The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14 a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53 and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   CDC14B
Gene Name:   CDC14 cell division cycle 14 homolog B (S. cerevisiae)
Aliases:   CDC14B3, Cdc14B1, Cdc14B2, hCDC14B
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000081377
Entrez:   8555
UniGene:   Hs.40582
Chromosome Mapping:   9q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.780000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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