PrimePCR™ Probe Assay: MKRN2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MKRN2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MKRN2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049640
Assay Design:   exonic
Chromosome Location:   3:12623846-12623951question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000170447 ENST00000411987 ENST00000448482

Gene Information

Members of the makorin family including MKRN2 have a characteristic zinc finger composition that suggests that they are ribonucleoproteins (Gray et al. 2001 [PubMed 11597136]).[supplied by OMIM Mar 2008]

Gene Symbol:   MKRN2
Gene Name:   makorin ring finger protein 2
Aliases:   RNF62
RefSeq:   NC_000003.11 NG_007467.1 NT_022517.18
Ensembl:   ENSG00000075975
Entrez:   23609
Chromosome Mapping:   3p25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.380000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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