PrimePCR™ Probe Assay: WDR62, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: WDR62, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WDR62, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049637
Assay Design:   exonic
Chromosome Location:   19:36558760-36558892question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000378860 ENST00000401500 ENST00000270301 ENST00000427823 ENST00000388999

Gene Information

This gene is proposed to play a role in cerebral cortical development. Mutations in this gene have been associated with microencephaly cortical malformations and mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jan 2011]

Gene Symbol:   WDR62
Gene Name:   WD repeat domain 62
Aliases:   C19orf14, DKFZp434J046, DKFZp686G1024, FLJ33298, MCPH2, MGC166976
RefSeq:   NC_000019.9 NG_028101.1 NT_011109.16
Ensembl:   ENSG00000075702
Entrez:   284403
Chromosome Mapping:   19q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.280000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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