PrimePCR™ Probe Assay: DHRS9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DHRS9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DHRS9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049602
Assay Design:   exonic
Chromosome Location:   2:169952066-169952215question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000602501 ENST00000357546 ENST00000428522 ENST00000421653 ENST00000436483 ENST00000432060 ENST00000327239 ENST00000412271

Gene Information

Description Not Available

Gene Symbol:   DHRS9
Gene Name:   dehydrogenase/reductase (SDR family) member 9
Aliases:   3ALPHA-HSD, RDH15, RDHL, RDHTBE, RETSDR8, SDR9C4
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000073737
Entrez:   10170
Chromosome Mapping:   2q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.970000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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