PrimePCR™ Probe Assay: ROGDI, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ROGDI, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ROGDI, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049527
Assay Design:   exonic
Chromosome Location:   16:4847738-4847836question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000512702 ENST00000503292 ENST00000389652 ENST00000424120 ENST00000413206 ENST00000367511 ENST00000474559 ENST00000378387 ENST00000378395 ENST00000343259 ENST00000322048 ENST00000592019 ENST00000586504 ENST00000591392 ENST00000587711 ENST00000586153 ENST00000355303 ENST00000316296 ENST00000530676 ENST00000531349 ENST00000538023 ENST00000398543 ENST00000261745

Gene Information

This gene encodes a protein of unknown function. Loss-of-function mutation in this gene cause Kohlschutter-Tonz syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq Mar 2012]

Gene Symbol:   ROGDI
Gene Name:   rogdi homolog (Drosophila)
Aliases:   FLJ22386
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000067836
Entrez:   79641
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.350000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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