PrimePCR™ Probe Assay: ACSM2B, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049504
Assay Design:   exonic
Chromosome Location:   16:20570677-20576003question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000329697 ENST00000565322 ENST00000565232 ENST00000567001 ENST00000568882 ENST00000566384 ENST00000569344 ENST00000414188 ENST00000324096 ENST00000544059 ENST00000318636 ENST00000574251 ENST00000576361 ENST00000417235 ENST00000573854 ENST00000219054 ENST00000575690 ENST00000571894 ENST00000396104 ENST00000424070 ENST00000536134

Gene Information

Description Not Available

Gene Symbol:   ACSM2B
Gene Name:   acyl-CoA synthetase medium-chain family member 2B
Aliases:   ACSM2, HXMA
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000066813
Entrez:   348158
UniGene:   Hs.567879
Chromosome Mapping:   16p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 35.040000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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