PrimePCR™ Probe Assay: HEXB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HEXB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HEXB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049387
Assay Design:   exonic
Chromosome Location:   5:74016338-74016567question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000511181 ENST00000261416 ENST00000513336 ENST00000509579

Gene Information

Hexosaminidase B is the beta subunit of the lysosomal enzyme beta-hexosaminidase that together with the cofactor GM2 activator protein catalyzes the degradation of the ganglioside GM2 and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits alpha and beta which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). [provided by RefSeq Jul 2008]

Gene Symbol:   HEXB
Gene Name:   hexosaminidase B (beta polypeptide)
Aliases:   ENC-1AS
RefSeq:   NC_000005.9 NG_009770.1 NG_011531.1 NT_006713.15
Ensembl:   ENSG00000049860
Entrez:   3074
Chromosome Mapping:   5q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.490000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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