PrimePCR™ Probe Assay: HSPA5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HSPA5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HSPA5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049360
Assay Design:   exonic
Chromosome Location:   9:127997895-127998015question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000324460

Gene Information

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER) and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins it may play a key role in monitoring protein transport through the cell.[provided by RefSeq Sep 2010]

Gene Symbol:   HSPA5
Gene Name:   heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Aliases:   BIP, FLJ26106, GRP78, MIF2
RefSeq:   NC_000009.11 NG_027761.1 NT_008470.19
Ensembl:   ENSG00000044574
Entrez:   3309
Chromosome Mapping:   9q33.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.640000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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