PrimePCR™ Probe Assay: OTC, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049327
Assay Design:   exonic
Chromosome Location:   X:38280355-38280497question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000039007

Gene Information

This nuclear gene encodes a mitochondrial matrix enzyme. Missense nonsense and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy it may play a role in that disease also. [provided by RefSeq Jul 2008]

Gene Symbol:   OTC
Gene Name:   ornithine carbamoyltransferase
Aliases:   MGC129967, MGC129968, MGC138856, OCTD
RefSeq:   NC_000023.10 NG_008471.1 NT_079573.4
Ensembl:   ENSG00000036473
Entrez:   5009
UniGene:   Hs.117050
Chromosome Mapping:   Xp21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.060000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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