PrimePCR™ Probe Assay: SLC4A7, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049316
Assay Design:   exonic
Chromosome Location:   3:27436118-27436226question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000419036 ENST00000295736 ENST00000428386 ENST00000454389 ENST00000440156 ENST00000437179 ENST00000446700 ENST00000455077 ENST00000445684 ENST00000428179 ENST00000435667 ENST00000388777 ENST00000425128

Gene Information

Description Not Available

Gene Symbol:   SLC4A7
Gene Name:   solute carrier family 4, sodium bicarbonate cotransporter, member 7
Aliases:   DKFZp686H168, NBC2, NBC3, SBC2, SLC4A6
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000033867
Entrez:   9497
UniGene:   Hs.250072
Chromosome Mapping:   3p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.010000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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