PrimePCR™ Probe Assay: B4GALT7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: B4GALT7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: B4GALT7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049301
Assay Design:   exonic
Chromosome Location:   5:177031427-177031523question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000409318 ENST00000376840 ENST00000358894 ENST00000422202 ENST00000326996 ENST00000435874 ENST00000561623 ENST00000356183 ENST00000487664 ENST00000371757 ENST00000486577 ENST00000491806 ENST00000488444 ENST00000490355 ENST00000298480 ENST00000263604 ENST00000279028 ENST00000328333 ENST00000306077 ENST00000425359 ENST00000436361 ENST00000440596 ENST00000302328 ENST00000444237 ENST00000450244 ENST00000394968 ENST00000330120 ENST00000276297 ENST00000358919 ENST00000512044 ENST00000520226 ENST

Gene Information

This gene is a member of the beta-14-galactosyltransferase (beta4GalT) family. Family members encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose. Each beta4GalT member has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus which then remains uncleaved to function as a transmembrane anchor. The enzyme encoded by this gene attaches the first galactose in the common carbohydrate-protein linkage (GlcA-beta13-Gal-beta13-Gal-beta14-Xyl-beta1-O-Ser) found in proteoglycans. This enzyme differs from other beta4GalTs because it lacks the conserved Cys residues found in beta4GalT1-beta4GalT6 and it is located in cis-Golgi instead of trans-Golgi. Mutations in this gene have been associated with the progeroid form of Ehlers-Danlos syndrome. [provided by RefSeq Oct 2009]

Gene Symbol:   B4GALT7
Gene Name:   xylosylprotein beta 1,4-galactosyltransferase, polypeptide 7 (galactosyltransferase I)
Aliases:   XGALT1, XGPT1
RefSeq:   NC_000005.9 NG_015977.1 NT_023133.13
Ensembl:   ENSG00000027847
Entrez:   11285
Chromosome Mapping:   5q35.2-q35.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.770000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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