PrimePCR™ Probe Assay: ZNF839, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF839, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF839, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049284
Assay Design:   exonic
Chromosome Location:   14:102805453-102805559question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000558850 ENST00000559185 ENST00000442396 ENST00000561251 ENST00000559155 ENST00000558490 ENST00000558462 ENST00000560112 ENST00000262236

Gene Information

Description Not Available

Gene Symbol:   ZNF839
Gene Name:   zinc finger protein 839
Aliases:   C14orf131, FLJ11132
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000022976
Entrez:   55778
Chromosome Mapping:   14q32.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.570000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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