PrimePCR™ Probe Assay: MAGIX, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MAGIX, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAGIX, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049269
Assay Design:   exonic
Chromosome Location:   X:49021415-49021642question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000554823 ENST00000331628 ENST00000376339 ENST00000425661 ENST00000458388 ENST00000412696 ENST00000415364 ENST00000376338 ENST00000425285 ENST00000454342 ENST00000595224

Gene Information

Description Not Available

Gene Symbol:   MAGIX
Gene Name:   MAGI family member, X-linked
Aliases:   FLJ21687, JM10, MGC138889, PDZX
RefSeq:   NC_000023.10 NG_016420.1 NT_079573.4
Ensembl:   ENSG00000017621
Entrez:   79917
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.360000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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