PrimePCR™ Probe Assay: SLC38A5, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049268
Assay Design:   exonic
Chromosome Location:   X:48324469-48324706question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000508239 ENST00000449771 ENST00000549151 ENST00000389212 ENST00000548919 ENST00000405493 ENST00000171000 ENST00000529022 ENST00000292524 ENST00000344063 ENST00000297788 ENST00000494552 ENST00000464672 ENST00000298532 ENST00000517309 ENST00000455950 ENST00000376876 ENST00000376875 ENST00000440085 ENST00000441948 ENST00000413668 ENST00000416711 ENST00000429543 ENST00000317669 ENST00000228606 ENST00000291574 ENST00000264741 ENST00000230124 ENST00000369451 ENST00000570156 ENST00000441106 ENST

Gene Information

The protein encoded by this gene is a system N sodium-coupled amino acid transporter involved in the transfer of glutamine asparagine histidine serine alanine and glycine. The encoded protein does not transport charged amino acids imino acids or N-alkylated amino acids. This transporter is not inhibited by lithium.[provided by RefSeq Jun 2010]

Gene Symbol:   SLC38A5
Gene Name:   solute carrier family 38, member 5
Aliases:   JM24, SN2, SNAT5, pp7194
RefSeq:   NC_000023.10 NG_021469.1 NT_079573.4
Ensembl:   ENSG00000017483
Entrez:   92745
UniGene:   Hs.195155
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.610000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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