PrimePCR™ Probe Assay: CD22, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049244
Assay Design:   exonic
Chromosome Location:   19:35823555-35823671question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000598537 ENST00000600131 ENST00000595780 ENST00000597916 ENST00000593867 ENST00000600424 ENST00000599811 ENST00000536635 ENST00000085219 ENST00000544992 ENST00000419549 ENST00000594250 ENST00000341773 ENST00000270311

Gene Information

Description Not Available

Gene Symbol:   CD22
Gene Name:   CD22 molecule
Aliases:   FLJ22814, MGC130020, SIGLEC-2, SIGLEC2
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000012124
Entrez:   933
UniGene:   Hs.579691
Chromosome Mapping:   19q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.560000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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