PrimePCR™ Probe Assay: CD4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CD4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CD4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049225
Assay Design:   exonic
Chromosome Location:   12:6898714-6898803question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000381775 ENST00000520584 ENST00000522452 ENST00000373907 ENST00000339266 ENST00000444555 ENST00000275603 ENST00000540286 ENST00000289957 ENST00000373016 ENST00000379873 ENST00000357991 ENST00000357544 ENST00000379869 ENST00000360279 ENST00000356606 ENST00000340581 ENST00000379878 ENST00000354791 ENST00000379876 ENST00000372827 ENST00000361413 ENST00000590577 ENST00000011653 ENST00000541982 ENST00000539492 ENST00000435200 ENST00000594128 ENST00000600498 ENST00000600809 ENST00000595745 ENST

Gene Information

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes but also in B cells macrophages and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq Aug 2010]

Gene Symbol:   CD4
Gene Name:   CD4 molecule
Aliases:   CD4mut
RefSeq:   NC_000012.11 NG_027688.1 NT_009759.16
Ensembl:   ENSG00000010610
Entrez:   920
Chromosome Mapping:   12pter-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.300000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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