PrimePCR™ Probe Assay: DYRK4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DYRK4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DYRK4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049220
Assay Design:   exonic
Chromosome Location:   12:4721751-4721849question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000542744 ENST00000540757 ENST00000543431 ENST00000544671 ENST00000010132 ENST00000545342

Gene Information

Dual-specificity kinases such as DYRK4 play key roles in cell proliferation survival and development (Zhang et al. 2005 [PubMed 15607427]).[supplied by OMIM Mar 2008]

Gene Symbol:   DYRK4
Gene Name:   dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4
Aliases:   Not Available
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000010219
Entrez:   8798
Chromosome Mapping:   12p13.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.870000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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