PrimePCR™ Probe Assay: RAD52, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049135
Assay Design:   exonic
Chromosome Location:   12:1040473-1042227question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000358495 ENST00000430095 ENST00000536177 ENST00000542785 ENST00000397230 ENST00000544742 ENST00000541619 ENST00000545564

Gene Information

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52 a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51 which suggested its role in RAD51 related DNA recombination and repair. [provided by RefSeq Jul 2008]

Gene Symbol:   RAD52
Gene Name:   RAD52 homolog (S. cerevisiae)
Aliases:   Not Available
RefSeq:   NC_000012.11 NG_007984.2 NG_017078.1 NT_009759.16
Ensembl:   ENSG00000002016
Entrez:   5893
UniGene:   Hs.410355
Chromosome Mapping:   12p13-p12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 35.990000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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