PrimePCR™ Probe Assay: ACY1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: ACY1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ACY1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041864
Assay Design:   exonic
Chromosome Location:   3:52023055-34817421question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000340908 ENST00000530271 ENST00000537687 ENST00000315939 ENST00000535572 ENST00000300648 ENST00000534313 ENST00000527525 ENST00000394224 ENST00000394227 ENST00000445101 ENST00000431721 ENST00000446860 ENST00000443964 ENST00000332271 ENST00000263253 ENST00000522555 ENST00000522236 ENST00000316059 ENST00000263801 ENST00000382039 ENST00000382044 ENST00000450115 ENST00000522455 ENST00000518566 ENST00000517638 ENST00000523850 ENST00000521376 ENST00000476854 ENST00000476351 ENST00000494103 ENST

Gene Information

Description Not Available

Gene Symbol:   ACY1
Gene Name:   aminoacylase 1
Aliases:   ACY-1, ACY1D
RefSeq:   NC_000003.11 NG_012036.1 NT_022517.18
Ensembl:   ENSG00000243989
Entrez:   95
Chromosome Mapping:   3p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.210000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download