PrimePCR™ Probe Assay: SCNN1D, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SCNN1D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SCNN1D, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041722
Assay Design:   exonic
Chromosome Location:   1:1223101-1223195question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000568392 ENST00000570268 ENST00000373857 ENST00000539896 ENST00000353088 ENST00000476071 ENST00000216879 ENST00000350991 ENST00000381658 ENST00000417954 ENST00000528838 ENST00000400780 ENST00000394413 ENST00000508545 ENST00000394414 ENST00000394411 ENST00000336640 ENST00000504198 ENST00000394409 ENST00000356826 ENST00000453001 ENST00000394410 ENST00000308508 ENST00000379116 ENST00000338555 ENST00000325425 ENST00000379099 ENST00000400928 ENST00000373657 ENST00000334463 ENST00000534715 ENST

Gene Information

Description Not Available

Gene Symbol:   SCNN1D
Gene Name:   sodium channel, nonvoltage-gated 1, delta
Aliases:   ENaCd, ENaCdelta, MGC149710, MGC149711, SCNED, dNaCh
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000162572
Entrez:   6339
Chromosome Mapping:   1p36.3-p36.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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