PrimePCR™ Probe Assay: LILRA4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LILRA4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LILRA4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041706
Assay Design:   exonic
Chromosome Location:   19:54844838-54844981question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000358127 ENST00000305641 ENST00000396365 ENST00000291759 ENST00000251377 ENST00000251376 ENST00000391737 ENST00000391738 ENST00000251372 ENST00000453777 ENST00000576226 ENST00000571783 ENST00000573976 ENST00000570811 ENST00000577050 ENST00000573402 ENST00000575013 ENST00000576301 ENST00000570657 ENST00000574047 ENST00000575706 ENST00000575950 ENST00000577069 ENST00000576139 ENST00000571445 ENST00000574300

Gene Information

This gene encodes an immunoglobulin-like cell surface protein preferentially expressed in plasmacytoid dendritic cells (PDCs). This gene is highly expressed in PDCs and is found to be rapidly down-regulated by interleukin 3 (IL3). This gene is one of the 19 highly related genes that form a leukocyte receptor gene cluster (LRC) at chromosomal region 19q13.4. [provided by RefSeq Jul 2008]

Gene Symbol:   LILRA4
Gene Name:   leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4
Aliases:   CD85g, ILT7, MGC129597, MGC129598
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000239961
Entrez:   23547
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.510000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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