PrimePCR™ Probe Assay: AL162731.1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: AL162731.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AL162731.1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041704
Assay Design:   exonic
Chromosome Location:   9:46390441-46390560question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000361360 ENST00000418388 ENST00000561457 ENST00000406506 ENST00000418795 ENST00000441768 ENST00000377529 ENST00000377525 ENST00000417276 ENST00000426006 ENST00000455764 ENST00000561204 ENST00000446041 ENST00000282470 ENST00000418378 ENST00000318037

Gene Information

Description Not Available

Gene Symbol:   AL162731.1
Gene Name:   Protein FAM27D1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000231997
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.630000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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