PrimePCR™ Probe Assay: DPPA3, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041690
Assay Design:   exonic
Chromosome Location:   12:7864118-7867781question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000262605 ENST00000372904 ENST00000345088 ENST00000419165

Gene Information

This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice this gene may play a role in transcriptional repression cell division and maintenance of cell pluripotentiality. In humans related intronless loci are located on chromosomes 14 and X. [provided by RefSeq Jul 2008]

Gene Symbol:   DPPA3
Gene Name:   developmental pluripotency associated 3
Aliases:   STELLA
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000187569
Entrez:   359787
UniGene:   Hs.131358
Chromosome Mapping:   12p13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.510000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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