This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: DPPA3, Human
PrimePCR™ Template for Probe Assay: DPPA3, Human
This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice this gene may play a role in transcriptional repression cell division and maintenance of cell pluripotentiality. In humans related intronless loci are located on chromosomes 14 and X. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.