PrimePCR™ Probe Assay: CSH1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CSH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CSH1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041651
Assay Design:   exonic
Chromosome Location:   17:61972354-61972490question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000266069 ENST00000449787 ENST00000456543 ENST00000423893 ENST00000332800 ENST00000316193 ENST00000329882 ENST00000453363 ENST00000561003 ENST00000259003 ENST00000438387 ENST00000346606 ENST00000450719 ENST00000351388 ENST00000458650 ENST00000323322 ENST00000392886 ENST00000336844 ENST00000560142 ENST00000345366 ENST00000394979 ENST00000258530 ENST00000539978 ENST00000551662 ENST00000294753 ENST00000461277 ENST00000366498

Gene Information

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites. Expression of the identical mature proteins for chorionic somatomammotropin hormones 1 and 2 is upregulated during development although the ratio of 1 to 2 increases by term. Mutations in this gene result in placental lactogen deficiency and Silver-Russell syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   CSH1
Gene Name:   chorionic somatomammotropin hormone 1 (placental lactogen)
Aliases:   CS-1, CSA, CSMT, FLJ75407, PL, hCS-A
RefSeq:   NC_000017.10 NG_001334.1 NT_010783.15
Ensembl:   ENSG00000136488
Entrez:   1442
Chromosome Mapping:   17q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.040000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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