PrimePCR™ Probe Assay: LSM5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LSM5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LSM5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041637
Assay Design:   exonic
Chromosome Location:   7:32526753-32527322question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000383836 ENST00000360413 ENST00000409709 ENST00000409893 ENST00000458637 ENST00000409619 ENST00000457074 ENST00000413503 ENST00000338508 ENST00000370018 ENST00000508582 ENST00000360203 ENST00000318100 ENST00000371600 ENST00000533211 ENST00000529997 ENST00000309996 ENST00000359895 ENST00000355841 ENST00000356618 ENST00000371817 ENST00000429492 ENST00000220616 ENST00000519178 ENST00000519543 ENST00000377869 ENST00000542445 ENST00000359499 ENST00000450169 ENST00000409909 ENST00000409292 ENST

Gene Information

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles which are important for pre-mRNA splicing.[supplied by OMIM Apr 2004]

Gene Symbol:   LSM5
Gene Name:   LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Aliases:   FLJ12710, YER146W
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000106355
Entrez:   23658
Chromosome Mapping:   7p14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.620000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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