PrimePCR™ Probe Assay: HSP90AB1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041572
Assay Design:   exonic
Chromosome Location:   6:44221352-44221499question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000371646 ENST00000353801 ENST00000371554

Gene Information

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction protein folding protein degradation and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins HSP90AA1 (MIM 140571) an inducible form and HSP90AB1 a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al. 2005 [PubMed 16269234]).[supplied by OMIM Aug 2008]

Gene Symbol:   HSP90AB1
Gene Name:   heat shock protein 90kDa alpha (cytosolic), class B member 1
Aliases:   D6S182, FLJ26984, HSP90-BETA, HSP90B, HSPC2, HSPCB
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000096384
Entrez:   3326
UniGene:   Hs.509736
Chromosome Mapping   6p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.640000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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