PrimePCR™ Probe Assay: RBCK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RBCK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RBCK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041570
Assay Design:   exonic
Chromosome Location:   20:389341-390550question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000411647 ENST00000356286 ENST00000475269 ENST00000441733 ENST00000353660 ENST00000382181 ENST00000400247

Gene Information

The protein encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus resulting in distinct isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   RBCK1
Gene Name:   RanBP-type and C3HC4-type zinc finger containing 1
Aliases:   C20orf18, HOIL-1, HOIL1, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000125826
Entrez:   10616
Chromosome Mapping:   20p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.600000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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