PrimePCR™ Probe Assay: EEF1G, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: EEF1G, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EEF1G, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041559
Assay Design:   exonic
Chromosome Location:   11:62334278-62334385question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000322344 ENST00000596014 ENST00000600910 ENST00000600573 ENST00000317961 ENST00000382806 ENST00000440077 ENST00000541639 ENST00000040738 ENST00000329251 ENST00000378019 ENST00000230671 ENST00000524041 ENST00000366637 ENST00000439617 ENST00000406207 ENST00000298552 ENST00000440111 ENST00000545250

Gene Information

This gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. [provided by RefSeq Jul 2008]

Gene Symbol:   EEF1G
Gene Name:   eukaryotic translation elongation factor 1 gamma
Aliases:   EF1G, GIG35
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000254772
Entrez:   1937
Chromosome Mapping:   11q12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 37.020000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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