PrimePCR™ Probe Assay: HLA-DPB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HLA-DPB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HLA-DPB1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041543
Assay Design:   exonic
Chromosome Location:   HSCHR6_MHC_COX:32976475-32976658question
Amplicon Length:   154
Splice Variants Targeted:   ENST00000433800 ENST00000444931 ENST00000447674 ENST00000552574 ENST00000411749 ENST00000441981 ENST00000411722 ENST00000418931 ENST00000416804 ENST00000454006 ENST00000411424 ENST00000458614 ENST00000548235 ENST00000425130 ENST00000411558 ENST00000455122 ENST00000383102 ENST00000441857 ENST00000434121 ENST00000548608 ENST00000551599 ENST00000415996 ENST00000447783 ENST00000445925 ENST00000548074 ENST00000548907 ENST00000432661 ENST00000399491 ENST00000399500

Gene Information

Description Not Available

Gene Symbol:   HLA-DPB1
Gene Name:   major histocompatibility complex, class II, DP beta 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000230708
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.270000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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