PrimePCR™ Probe Assay: C1QTNF9B, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041525
Assay Design:   exonic
Chromosome Location:   13:24465429-24465526question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000303688 ENST00000383794 ENST00000416649 ENST00000294129 ENST00000413374 ENST00000341520 ENST00000317506 ENST00000319778 ENST00000375110 ENST00000263062 ENST00000341584 ENST00000277541 ENST00000402558 ENST00000379174 ENST00000379166 ENST00000345050 ENST00000377648 ENST00000361874 ENST00000409438 ENST00000409240 ENST00000409868 ENST00000409567 ENST00000394003 ENST00000407639 ENST00000382145 ENST00000382140 ENST00000382137 ENST00000382396 ENST00000397272 ENST00000382071 ENST00000332018 ENST

Gene Information

Description Not Available

Gene Symbol:   C1QTNF9B
Gene Name:   C1QTNF9B
Aliases:   CTRP9B, MGC117159
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000205863
Entrez:   387911
UniGene:   Hs.642624
Chromosome Mapping:   13q12.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.520000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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