PrimePCR™ Probe Assay: LCN12, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LCN12, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LCN12, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041493
Assay Design:   exonic
Chromosome Location:   9:139846784-139846892question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000320736 ENST00000445522 ENST00000447234 ENST00000373164 ENST00000244565 ENST00000371633 ENST00000300591 ENST00000579038 ENST00000582408 ENST00000441551 ENST00000398722 ENST00000536736 ENST00000441893 ENST00000360890 ENST00000325239 ENST00000413659 ENST00000348624 ENST00000360614 ENST00000585374 ENST00000593119 ENST00000540670 ENST00000590729 ENST00000331569 ENST00000536441 ENST00000265641 ENST00000372437 ENST00000372408 ENST00000457953 ENST00000372421 ENST00000404771 ENST00000372440 ENST

Gene Information

Members of the lipocalin family such as LCN12 have a common structure consisting of an 8-stranded antiparallel beta-barrel that forms a cup-shaped ligand-binding pocket or calyx. Lipocalins generally bind small hydrophobic ligands and transport them to specific cells (Suzuki et al. 2004 [PubMed 15363845]).[supplied by OMIM Aug 2009]

Gene Symbol:   LCN12
Gene Name:   lipocalin 12
Aliases:   MGC34753, MGC48935
RefSeq:   NC_000009.11 NT_024000.16
Ensembl:   ENSG00000184925
Entrez:   286256
Chromosome Mapping:   9q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.260000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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