PrimePCR™ Probe Assay: EWSR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EWSR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EWSR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041492
Assay Design:   exonic
Chromosome Location:   22:29669730-29669819question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000586471 ENST00000300961 ENST00000593238 ENST00000444626 ENST00000397938 ENST00000436425 ENST00000447973 ENST00000406548 ENST00000437155 ENST00000331029 ENST00000414183 ENST00000333395 ENST00000455726 ENST00000332035 ENST00000332050 ENST00000334414 ENST00000451516 ENST00000535581

Gene Information

This gene encodes a multifunctional protein that is involved in various cellular processes including gene expression cell signaling and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene specifically a t(11;22)(q24;q12) translocation are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq Jul 2009]

Gene Symbol:   EWSR1
Gene Name:   Ewing sarcoma breakpoint region 1
Aliases:   EWS, bK984G1.4
RefSeq:   NC_000022.10 NT_011520.12 NG_023240.1
Ensembl:   ENSG00000182944
Entrez:   2130
Chromosome Mapping:   22q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.060000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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