PrimePCR™ Probe Assay: WASH6P, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041490
Assay Design:   exonic
Chromosome Location:   X:155255018-155255151question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000535845 ENST00000538924 ENST00000541910 ENST00000165698 ENST00000539981 ENST00000326592 ENST00000359512 ENST00000285718 ENST00000379619 ENST00000264716

Gene Information

Description Not Available

Gene Symbol:   WASH6P
Gene Name:   WAS protein family homolog 6 pseudogene
Aliases:   CXYorf1, FAM39A, FLJ77647, WASH
RefSeq:   NC_000023.10 NC_000024.9 NG_008380.2 NT_167198.1 NT_167206.1
Ensembl:   ENSG00000182484
Entrez:   653440
UniGene:   Not Available
Chromosome Mapping:   Xq28;Yq12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.710000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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