PrimePCR™ Probe Assay: KCNK7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KCNK7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KCNK7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041475
Assay Design:   exonic
Chromosome Location:   11:65360504-65360615question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000525254 ENST00000530380 ENST00000340313 ENST00000394216 ENST00000394217 ENST00000342202 ENST00000393359 ENST00000341184 ENST00000315266 ENST00000478722 ENST00000543237 ENST00000305960 ENST00000369774 ENST00000355946 ENST00000377306 ENST00000451767 ENST00000277865 ENST00000537649 ENST00000373886 ENST00000319004 ENST00000576778 ENST00000520356 ENST00000520727 ENST00000429442 ENST00000377838 ENST00000520214 ENST00000523399 ENST00000243300 ENST00000428173

Gene Information

This gene encodes a member of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel; however it may require other non-pore-forming proteins for activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   KCNK7
Gene Name:   potassium channel, subfamily K, member 7
Aliases:   K2p7.1, MGC118782, MGC118784, TWIK3
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000173338
Entrez:   10089
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.200000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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