PrimePCR™ Probe Assay: TTC36, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TTC36, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TTC36, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041473
Assay Design:   exonic
Chromosome Location:   11:118399318-118399452question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000562968 ENST00000357736 ENST00000302783 ENST00000539546 ENST00000368061 ENST00000263635 ENST00000454300 ENST00000369831 ENST00000460717 ENST00000241337 ENST00000414179 ENST00000369829 ENST00000442650 ENST00000536255 ENST00000381209 ENST00000381208 ENST00000371817 ENST00000565691 ENST00000335950 ENST00000556526 ENST00000394071 ENST00000286523 ENST00000458379 ENST00000589918 ENST00000303225 ENST00000589620 ENST00000353139 ENST00000360679 ENST00000394406 ENST00000335166 ENST00000296292 ENST

Gene Information

Description Not Available

Gene Symbol:   TTC36
Gene Name:   tetratricopeptide repeat domain 36
Aliases:   HBP21
RefSeq:   NC_000011.9 NG_027813.1 NT_033899.8
Ensembl:   ENSG00000172425
Entrez:   143941
Chromosome Mapping:   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.930000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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