PrimePCR™ Probe Assay: CES5A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CES5A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CES5A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041447
Assay Design:   exonic
Chromosome Location:   16:55880298-55880443question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000372045 ENST00000434224 ENST00000218054 ENST00000369485 ENST00000265607 ENST00000538899 ENST00000521992 ENST00000319165 ENST00000518005 ENST00000290567 ENST00000520435

Gene Information

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics such as cocaine and heroin and endogenous substrates with ester thioester or amide bonds. They also participate in fatty acyl and cholesterol ester metabolism and may play a role in the blood-brain barrier system. This gene also called CES5 is predominantly expressed in peripheral tissues including brain kidney lung and testis. It encodes a secreted enzyme. Because of high levels in the urine of male domestic cats this enzyme is also called cauxin (carboxylesterase-like urinary excreted protein). The enzyme functions in regulating the production of a pheromone precursor and may contribute to lipid and cholesterol transfer processes within male reproductive fluids. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jun 2010]

Gene Symbol:   CES5A
Gene Name:   carboxylesterase 5A
Aliases:   CAUXIN, CES4C1, CES5, CES7, FLJ31547
RefSeq:   NC_000016.9 NT_010498.15 NW_003315945.1
Ensembl:   ENSG00000159398
Entrez:   221223
Chromosome Mapping:   16q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.720000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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