PrimePCR™ Probe Assay: PRSS53, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PRSS53, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRSS53, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041440
Assay Design:   exonic
Chromosome Location:   16:31097287-31097426question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000265068 ENST00000331343 ENST00000398932 ENST00000438657 ENST00000398930 ENST00000279027 ENST00000472148 ENST00000495082 ENST00000413164 ENST00000290317 ENST00000396360 ENST00000435032 ENST00000289013 ENST00000382839 ENST00000324666 ENST00000502458 ENST00000505311 ENST00000513350 ENST00000545951 ENST00000567020 ENST00000564879 ENST00000280606 ENST00000373091 ENST00000373093 ENST00000395209 ENST00000541709 ENST00000328771 ENST00000518471 ENST00000356206 ENST00000443444 ENST00000290524 ENST

Gene Information

Description Not Available

Gene Symbol:   PRSS53
Gene Name:   protease, serine, 53
Aliases:   FLJ00289, POL3S, UNQ308
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000151006
Entrez:   339105
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.420000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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