PrimePCR™ Probe Assay: ZNF7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ZNF7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041437
Assay Design:   exonic
Chromosome Location:   8:146054863-146062783question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000376038 ENST00000348603 ENST00000371756 ENST00000400585 ENST00000400573 ENST00000262608 ENST00000273317 ENST00000396776 ENST00000532777 ENST00000446747 ENST00000525266 ENST00000325217 ENST00000533314 ENST00000527218 ENST00000529819 ENST00000528372 ENST00000528130 ENST00000325241 ENST00000301318 ENST00000258111 ENST00000424728 ENST00000343098 ENST00000257766 ENST00000403245 ENST00000457545 ENST00000454109 ENST00000400546 ENST00000284894 ENST00000598566 ENST00000593643 ENST00000602116 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF7
Gene Name:   zinc finger protein 7
Aliases:   FLJ38706, HF.16, KOX4, zf30
RefSeq:   NC_000008.10 NT_037704.5
Ensembl:   ENSG00000147789
Entrez:   7553
Chromosome Mapping:   8q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.440000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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