PrimePCR™ Probe Assay: ATP5G2, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041421
Assay Design:   exonic
Chromosome Location:   12:54058993-54059164question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000602871 ENST00000394349 ENST00000549164 ENST00000338662 ENST00000394156 ENST00000268603 ENST00000567934

Gene Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core F1 and the membrane-spanning component Fo comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha beta gamma delta and epsilon) assembled with a stoichiometry of 3 alpha 3 beta and single representatives of the gamma delta and epsilon subunits. The proton channel likely has nine subunits (a b c d e f g F6 and 8). There are three separate genes which encode subunit c of the proton channel and they specify precursors with different import sequences but identical mature proteins. The protein encoded by this gene is one of three precursors of subunit c. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene has multiple pseudogenes. [provided by RefSeq Jun 2010]

Gene Symbol:   ATP5G2
Gene Name:   ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C2 (subunit 9)
Aliases:   Not Available
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000135390
Entrez:   517
UniGene:   Hs.524464
Chromosome Mapping:   12q13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.540000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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